Background: Microbial lipids are produced by many oleaginous organisms including the well-characterized yeast Yarrowia lipolytica, which can be engineered for increased lipid yield by up-regulation of the lipid biosynthetic pathway and down-regulation or deletion of competing pathways. Results: We describe a strain engineering strategy centered on diacylglycerol acyltransferase (DGA) gene overexpression that applied combinatorial screening of overexpression and deletion genetic targets to construct a high lipid producing yeast biocatalyst. The resulting strain, NS432, combines overexpression of a heterologous DGA1 enzyme from Rhodosporidium toruloides, a heterlogous DGA2 enzyme from Claviceps purpurea, and deletion of the native TGL3 lipase regulator. These three genetic modifications, selected for their effect on lipid production, enabled a 77 % lipid content and 0.21 g lipid per g glucose yield in batch fermentation. In fed-batch glucose fermentation NS432 produced 85 g/L lipid at a productivity of 0.73 g/L/h. Conclusions: The yields, productivities, and titers reported in this study may further support the applied goal of costeffective, large -scale microbial lipid production for use as biofuels and biochemicals.
CITATION STYLE
Friedlander, J., Tsakraklides, V., Kamineni, A., Greenhagen, E. H., Consiglio, A. L., MacEwen, K., … Brevnova, E. E. (2016). Engineering of a high lipid producing Yarrowia lipolytica strain. Biotechnology for Biofuels, 9(1). https://doi.org/10.1186/s13068-016-0492-3
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