Evaluation of the adhesion of human dental pulp stem cells to differentendodontic biomaterials before and after setting

Abstract

Background. Stem cell-based treatment modalities have been potential strategies for tissue regenerationin many conditions. Several studies have evaluated the biologic properties of DPSCs and their efficacyin the treatment of a variety of diseases. The present study was undertaken to evaluate the adhesionbehavior of DPSCs on different endodontic materials before and after setting. Methods. The crowns of the selected teeth were removed, and the root canals were prepared andobturated with gutta-percha and AH26 sealer. A retrograde cavity was prepared at root ends. Differentmaterials were placed in the cavities. Then the samples were attached to the wells with the use of achemical glue. Dental pulp stem cells were allowed to proliferate to reach a count of 2 million andtransferred to -12well plates in association with a culture medium. Finally, the samples attached to thewells were exposed to the stem cells immersed in the culture medium before and after setting. Thenadhesion of the stem cells was evaluated using SEM. Results. The SEM results showed cellular adhesion in the samples containing CEM cement both beforeand after setting. The samples containing MTA Angelus and ProRoot MTA exhibited cellular adhesionbefore setting, with no cellular adhesion after setting. The samples containing AH26 and MTA Fillapexsealers exhibited cellular adhesion after setting, with no adhesion before setting. The samples containingsimvastatin exhibited no cellular adhesion before setting; this material had dissolved in the culturemedium after setting evaluation. Conclusion. The results of the present study showed that of all the materials tested, CEM cement hadthe highest capacity for dental pulp stem cell adhesion.