Evaluation of streptococcus mutans contamination of tooth brushes and their decontamination using various disinfectants - An in vitro study

Abstract

Purpose: The objective was to determine the level of contamination of tooth brushes by Streptococcus mutans using microbiological identification, to assess the bacterial contamination using SEM and to evaluate the efficacy of different disinfectant solutions in reducing the contamination of tooth brushes. Methods: 24 children used their tooth brushes once a day, for five consecutive days. The tooth brushes were then immersed into disinfectant solution for 20 hours. Group I -0.12% chlorhexidine gluconate, Group II- 1% sodium hypochlorite, Group III - 3% hydrogen peroxide, Group IV- Tap water. They were then placed into test tubes containing bacitracin sucrose solution for 3 to 4 days at 37°C. Streptococcus mutans colonies were assessed and the tooth brushes were submitted for SEM analysis. Results: There was no bacterial growth observed in Group I, II and III, Group IV showed Streptococcus mutans growth. SEM showed biofilm formation on tooth brush bristles. Conclusion: Immersion in 0.12% Chlorhexidine gluconate, 1% sodium hypochlorite, 3% hydrogen peroxide is efficient method for tooth brush disinfection. [ABSTRACT FROM AUTHOR]