Single-molecule force spectroscopy is a powerful tool to analyze the architecture and interaction of large macromolecular assemblies that are refractory to high-resolution structural interrogations. Here, we describe an optical tweezers–based platform for extracting the mechanical fingerprints of individual nucleosome arrays bound with chromatin-associated complexes, such as the Polycomb repressive complex 2 (PRC2). This platform comprehensively characterizes the diverse binding modes of PRC2 on chromatin, measures their mechanical strengths, and is broadly applicable to the studies of other epigenetic machineries.
CITATION STYLE
Leicher, R., & Liu, S. (2022). Probing the Interaction Between Chromatin and Chromatin-Associated Complexes with Optical Tweezers. In Methods in Molecular Biology (Vol. 2478, pp. 313–327). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2229-2_11
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