A combinatorial approach to circuit mapping in the mouse olfactory bulb

Abstract

Neurons form neural circuits through functional synapses. Inputs and outputs across these circuits contribute to the brain’s intricate mechanisms of information processing and behavioral responses. By combining acousto-optic deflector-based scanning microscopy with optogenetics, whole cell electrophysiological recordings, and transgenic viral delivery, researchers can now investigate and map relevant neural circuits with high spatial and cell type-specific precision. The goal of this review is to provide the reader with guidelines and methods for using a combinatorial approach toward circuit mapping in rodent brain tissue.