This protocol describes a strategy for analyzing phylogenetic Y-SNPs in a hierarchical multiplex assay by utilizing the SNaPshot ® Multiplex System. Step by step, the protocol assists in the appropriate selection of SNPs, the primer design, the set up of PCR/SBE reactions as well as in the analysis of the results. Furthermore, a forensic approach is highlighted, in which the most probable ancestry of an unknown male DNA is inferred by the geographical distribution of the assigned Y-SNP haplogroup. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Geppert, M., & Roewer, L. (2012). SNaPshot ® minisequencing analysis of multiple ancestry-informative Y-SNPs using capillary electrophoresis. Methods in Molecular Biology, 830, 127–140. https://doi.org/10.1007/978-1-61779-461-2_9
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