Protein phosphorylation is one of the most important posttranslational modifications in cell signaling and regulation. Protein phosphorylation can be detected by site-specific phospho-antibodies, but generating these antibodies can be costly, time-consuming, and difficult. Recently, Phos-tag technology has been developed to detect protein phosphorylation. Here, we describe our method for using Phos-tag gels to compare the phosphorylation status of YAP and TAZ, the most important downstream effectors of the Hippo pathway.
CITATION STYLE
Chen, R., Plouffe, S. W., & Guan, K. L. (2019). Determining the Phosphorylation Status of Hippo Components YAP and TAZ Using Phos-tag. In Methods in Molecular Biology (Vol. 1893, pp. 281–287). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8910-2_21
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