Production of Cellulase and Xylanase from Eupenicillium Javanicum by Solid-State Fermentation Utilizing Pineapple Crown Leaves Waste as the Substrate

Abstract

Vast applications of cellulases and xylanases in many fields over the last few decades and high production cost demand extensive research in improving its quality and finding low cost substrates. These includes pulp and paper, food and feed, brewing, agriculture and biofuel industries. The main objective of this study was to investigate the use of pineapple crown leaves waste as the substrate to produce cellulases and xylanases in solid-state cultures (SSF) of Eupenicillium javanicum InaCC F154. Three SSF temperatures (25°C, 30°C and 35°C) and pH (4.0, 6.0 and 8.0) were tested for 96 h in solid media containing defatted soybean, (NH4)2SO4, NaNO3, CaCl2, and small amount of H2O. Then, its comparison with submerged fermentation (SmF) was also carried out. The SSF results showed that increasing temperature from 25°C to 35°C, increased the production of these enzymes. The pH also affected its activities, except at pH 4.0. The optimum pH and temperature for cellulase and xylanase production were found to be 6.0 and 35°C, respectively. The maximum cellulase obtained was 0.261 U/mL while much higher was obtained for xylanase with activity of 1.683 U/mL. SSF was better than SmF for xylanases (0.501 U/mL vs. 1.683 U/mL). The outcome of this study exhibits the potential of pineapple crown leaves as the substrate for xylanase production from E. javanicum by solid-state fermentation.