The viability of cell that encapsulated in calcium alginate hydrogel beads

Abstract

Objective: To prove that calcium alginate beads can be used as scaffolds during in vitro culture. Methods: Mouse preosteoblastic cells (MC3T3-E1) were encapsulated in calcium alginate hydrogel beads. The Cell Counting Kit-8 (CCK-8) assay was used to assess cell viability at 2, 5, 8, 11, 14, and 21 days. Calcein-AM and propidium iodide (PI) were employed for live/dead staining. Results: MC3T3-E1 cells were alive on day 21 and had the highest viability on day 14. Conclusion: MC3T3-E1 cells could be encapsulated in calcium alginate hydrogel beads and cultured. Calcium alginate hydrogel beads can be used as scaffolds for three-dimensional in vitro culture.