Current and future perspectives on isothermal nucleic acid amplification technologies for diagnosing infections

116Citations
Citations of this article
212Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Nucleic acid amplification technology (NAAT) has assumed a critical position in disease diagnosis in recent times and contributed significantly to healthcare. Application of these methods has resulted in a more sensitive, accurate and rapid diagnosis of infectious diseases than older traditional methods like culture-based identification. NAAT such as the polymerase chain reaction (PCR) is widely applied but seldom available to resource-limited settings. Isothermal amplification (IA) methods provide a rapid, sensitive, specific, simpler and less expensive procedure for detecting nucleic acid from samples. However, not all of these IA techniques find regular applications in infectious diseases diagnosis. Disease diagnosis and treatment could be improved, and the rapidly increasing problem of antimicrobial resistance reduced, with improvement, adaptation, and application of isothermal amplification methods in clinical settings, especially in developing countries. This review centres on some isothermal techniques that have found documented applications in infectious diseases diagnosis, highlighting their principles, development, strengths, setbacks and imminent potentials for use at points of care.

References Powered by Scopus

7203Citations
4360Readers

This article is free to access.

1792Citations
1579Readers
Get full text

Cited by Powered by Scopus

Your institution provides access to this article.

This article is free to access.

Your institution provides access to this article.

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Obande, G. A., & Singh, K. K. B. (2020). Current and future perspectives on isothermal nucleic acid amplification technologies for diagnosing infections. Infection and Drug Resistance. Dove Medical Press Ltd. https://doi.org/10.2147/IDR.S217571

Readers over time

‘20‘21‘22‘23‘24‘25020406080

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 56

62%

Researcher 19

21%

Professor / Associate Prof. 11

12%

Lecturer / Post doc 5

5%

Readers' Discipline

Tooltip

Biochemistry, Genetics and Molecular Bi... 46

55%

Agricultural and Biological Sciences 15

18%

Medicine and Dentistry 13

15%

Engineering 10

12%

Save time finding and organizing research with Mendeley

Sign up for free
0