Investigation of Penicillin Binding Protein (PBP)-like Peptide Cyclase and Hydrolase in Surugamide Non-ribosomal Peptide Biosynthesis

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Abstract

Non-ribosomal peptides (NRPs) are biosynthesized on non-ribosomal peptides synthetase (NRPS) complexes, of which a C-terminal releasing domain commonly offloads the products. Interestingly, a dedicated releasing domain is absent in surugamides (SGM) NRPS, which directs the biosynthesis of cyclic octapeptides, SGM-A to -E, and the linear decapeptide, SGM-F. Here, we confirmed that surE is essential for the production of SGMs via genetic experiments. Biochemical characterization demonstrated that the recombinant enzyme, SurE, can generate the main products SGM-A and -F from the corresponding SNAC substrates, indicating that SurE is a standalone thioesterase-like enzyme. SurE also displays considerable substrate plasticity with expanded ring or different amino acid compositions to produce different cyclopeptides, highlighting the potential of chemoenzymatic applications. Site-directed mutagenesis allowed identification of the key residues of SurE. Finally, bioinformatics analysis suggested that SurE homologs are widely distributed in bacteria, suggesting a general mechanism of NRP release in Nature. Zhou et al. reported a penicillin binding protein (PBP)-like thioesterase catalyzing peptide chain termination in surugamide non-ribosomal peptide (NRP) biosynthesis. The family of enzymes is widely distributed in NRP biosynthetic pathways in bacteria, either as standalones or C-terminal domains of multidomain NRPSs, suggesting a general mechanism in Nature.

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Zhou, Y., Lin, X., Xu, C., Shen, Y., Wang, S. P., Liao, H., … Lin, H. W. (2019). Investigation of Penicillin Binding Protein (PBP)-like Peptide Cyclase and Hydrolase in Surugamide Non-ribosomal Peptide Biosynthesis. Cell Chemical Biology, 26(5), 737-744.e4. https://doi.org/10.1016/j.chembiol.2019.02.010

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