Isolation of staphylococcus sp. RNA

Abstract

Isolation of RNA (ribonucleic acid) is a valuable technique to study gene regulation and functional RNAs. It is important to obtain pure samples of RNA for downstream applications, while avoiding the negative effects of ribonucleases (RNases). Here we describe several methods of extracting RNA, with an emphasis on Staphylococcus RNA isolation, and measures that must be taken to ensure high-quality, high-yield RNA. © Springer Science+Business Media, LLC 2014.