Prevalence of New Delhi Metallo-βLactamase-1 (blaNDM-1) Gene in Children from Tertiary Care Hospital of Pakistan

Abstract

Carbapenems are hydrolyzed by carbapanamase, present in the bacteria, which is a growing clinical threats. bla Ndm gene encodes for new Delhi metallo-beta lactamase, which can hydrolyze all types of beta-lactams. The objective of the study was to screen multiple drug resistant strains of bacteria for New delhi-metallo-beta-lactamase (bla-Ndm1) gene. Blood samples (5ml) of children suffering from different infections, under treatment in a teriary care hospital, were screened for blaNDM-1 gene. Blood samples of 116 patients having tested for multiple drug resistance were analyzed for blaNDM-1 gene by PCR. Sixteen samples were found to be positive for blaNDM-1 gene. The bacterial species harboring blaNDM-1 gene were 25% Enterobacter cloacae, 18.75% Klebsiella sp., 12.5% Pseudomonas sp., 12.5% Citrobacter freundii, 12.5% Acinetobacter Baumanii, 12.5% E. coli and 6.25% shigella sp. Nucleotide sequencing of PCR product of Klebsella sp, Enterobacter cloacea and Citrobacter freudii showed 100% sequence homology. It is concluded that there is high prevalence of blaNDM-1 among carbapenem resistant enterobacteriaceae isolated from patient suffering from different diseases at local tertiary care hospital of Lahore.