Investigation of Granzyme B (GrB) function and pathophysiology in both human settings and rodent models increasingly involve the use of indirect immunofluorescence imaging and fluorescence-activated cell sorting, which requires reliable GrB antibodies that do not recognise other closely related granzymes. Here, we describe the validation (using a set of recombinant granzymes, and GrB-deficient cells) and application of widely available monoclonal antibodies to specifically monitor GrB in human or mouse cells. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Bird, C. H., Hitchen, C., Prescott, M., Harper, I., & Bird, P. I. (2012). Immunodetection of Granzyme B tissue distribution and cellular localisation. Methods in Molecular Biology, 844, 237–250. https://doi.org/10.1007/978-1-61779-527-5_17
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