Pt Nanoparticles Confined by Zirconium Metal-Organic Frameworks with Enhanced Enzyme-like Activity for Glucose Detection

Abstract

Metal nanozymes hold promise for chemical and biological applications, and their implementation relies on high catalytic efficiency and stability. Using the metal-organic framework as an ideal carrier for well-dispersed ultra-small metal nanoparticles (NPs) is beneficial for improving the catalytic efficiency of nanozymes. In this study, a zirconium-based metal organic framework (UiO-66) with good chemical stability and high porosity was synthesized and used to construct Pt/UiO-66 nanocomposites. The percentage of Pt in UiO-66 can be tuned easily by adjusting the feeding amount of PtCl42-. Because of the confinement effect of mesopores, the Pt particles with an average diameter of 3.8 nm are formed and dispersed throughout the pores of the UiO-66 particle. The Pt/UiO-66 composites show efficient oxidase- and peroxidase-like activity. Both the oxidase- and peroxidase-like activities are dependent on the Pt percentage. Pt/UiO-66-6% exhibits enhanced peroxidase-like activity, ∼3.9 times higher than that of commercial Pt/C with 10 wt % Pt. We propose that the construction of Pt/UiO-66 increased the utilization efficiency and stability of Pt NPs and provided more active sites for catalytic reactions. Using the peroxidase-like activity of Pt/UiO-66, a colorimetric method that can be used for actual blood glucose detection was developed for the specific detection of glucose with a limit of detection of 0.033 mM.