Plasmids containing a site-specific DNA interstrand cross-link (ICL) are invaluable tools for the investigation of ICL repair pathways at the biochemical and cellular level. We describe a procedure for preparation of plasmid DNA substrates containing a single ICL at a specific site. The procedure is versatile, leads to reliable yields of pure DNA substrate, and is suitable for the incorporation of virtually any type of DNA lesion into plasmids. © 2012 Springer Science+Business Media New York.
CITATION STYLE
Enoiu, M., Ho, T. V., Long, D. T., Walter, J. C., & Schärer, O. D. (2012). Construction of plasmids containing site-specific DNA interstrand cross-links for biochemical and cell biological studies. Methods in Molecular Biology, 920, 203–219. https://doi.org/10.1007/978-1-61779-998-3_15
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