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Isolation of PolyA+ RNA

  • Surzycki S
Springer Berlin Heidelberg, (2000), 145-162
DOI: 10.1007/978-3-642-56968-5_7
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Abstract

Extraction, purification and analysis of mRNA are essential in understanding gene expression. Usually 1 to 3% ofcellular RNA is present as mRNA, the remaining being ribosomal RNA and various species ofsmall stable RNAs. For some applications, such as construction of cDNA libraries or analysis of low-abundance mRNAs, isolation of mRNA instead oftotal cellular RNA is essential.

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Surzycki, S. (2000). Isolation of PolyA+ RNA. In Basic Techniques in Molecular Biology (pp. 145–162). Springer Berlin Heidelberg. https://doi.org/10.1007/978-3-642-56968-5_7

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