Low-dose three-dimensional hard x-ray imaging of bacterial cells

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Abstract

We have imaged the three-dimensional density distribution of unstained and unsliced, freeze-dried cells of the gram-positive bacterium Deinococcus radiodurans by tomographic x-ray propagation microscopy, i.e. projection tomography with phase contrast formation by free space propagation. The work extends previous x-ray imaging of biological cells in the simple in-line holography geometry to full three-dimensional reconstruction, based on a fast iterative phase reconstruction algorithm which circumvents the usual twin-image problem. The sample is illuminated by the highly curved wave fronts emitted from a virtual quasi-point source with 10 nm cross section, realized by two crossed x-ray waveguides. The experimental scheme allows for a particularly dose efficient determination of the 3D density distribution in the cellular structure. © 2012 Bartels et al.

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Bartels, M., Priebe, M., Wilke, R. N., Krüger, S. P., Giewekemeyer, K., Kalbfleisch, S., … Salditt, T. (2012). Low-dose three-dimensional hard x-ray imaging of bacterial cells. Optical Nanoscopy, 1(1), 1–7. https://doi.org/10.1186/2192-2853-1-10

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