Study of tetrodotoxin detection in cultured medium

Abstract

Detection of TTX produced in cultured medium containing peptone, yeast extract that was made from animal or other biological sources caused the false positive results when using thin layer chromatgraphy method. With such results, it was a lot of mistakes when detect tetrodotoxin in food as a toxin or in medicine as a drug. Therefore, it was needed to look up a modification in TTX detection in cultured medium. In the study, the tested samples were designed and the mobile phases for thin layer chromatgraphy were optimized. The results showed the suitable system for TTX detection was pyridine: ethylacetate: acetic acid: chloroform with the ratio as 15:5:5:2 or butanol: acetic acid: distilled water (15:3:6). The tetrodotoxin level was detectable at 10 ng. © 2013 IFMBE.