Distribution and viral load of type specific HPVs in different cervical lesions as detected by PCR-ELISA

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Abstract

Aims - To investigate the distribution and viral load of the most prevalent high risk human papillomavirus (HPV) types 16, 18, 31, 33, and 45 and low risk HPV types 6 and 11 in a variety of cervical lesions. Methods - One hundred and seventy six cytological specimens from women with different cervical lesions were investigated. For an accurate standardisation of the sample, cervical cells were counted and a volume of the cell suspension processed by polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA). Semiquantitative determinations were achieved in relation to an external reference titration curve. Results - HPV DNA was detected in 60.2% of the samples. HPV-16 was the prevalent genotype (57.6%), followed by HPV-33, HPV-31, HPV-6, HPV-18, and HPV-45. HPV-11 was not detected. HPV-16 showed a pronounced increase in prevalence with the evolution of cervical disease. Semi-quantitative evaluation of the results showed that only HPV-16 DNA could reach very high values (> 1000 genome copies/cell) and a very high HPV-16 load correlated with the severity of cervical disease. Conclusions - Only HPV-16 load appears to be associated with the severity of cervical disease.

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APA

Zerbini, M., Venturoli, S., Cricca, M., Gallinella, G., De Simone, P., Costa, S., … Musiani, M. (2001). Distribution and viral load of type specific HPVs in different cervical lesions as detected by PCR-ELISA. Journal of Clinical Pathology, 54(5), 377–380. https://doi.org/10.1136/jcp.54.5.377

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