Studies regarding the optimization of the solvent consumption in the determination of organochlorine pesticides from complex fodder

Abstract

Since the main pathway for animal food contamination by OCPs is the ingestion of polluted feed, the determination of the contamination degree of the fodders is extremely important. In case of complex fodders in whose composition there are various materials, the potential contamination sources are multiple. The determination of organochlorine pesticides was made through the gas chromatography method using capillary columns and detector with electrons capture. For the separation step, a method specific to non-fat aliments was performed. This method requires the use of three subsequent elutions with 200 ml ethylic ether/light petroleum, in variable proportions. For the purpose of optimizing the method, there were determinations performed on the same samples through extraction in the normal variant and in reduced ones. This way, we could verify the level up to which the solvent consumption can be reduced without affecting the accuracy of the determination. The practical determinations proved that only the 1:2 reduced method has fully qualitative application and specific quantitative applications.