A novel controlled release system-based sandwich-type immunosensor is fabricated to detect squamous cell carcinoma antigen (SCCA). The 1-methyl-1H-benzimidazole functionalized mesoporous SiO 2 (MBI-MS) is used to load methylene blue (MB). β-cyclodextrin functionalized gold (CD-Au) is introduced as the gatekeeper for encapsulating MB and capturing the adamantly functional detection antibody (ADA-Ab 2). And pH stimulus serves as the trigger system to control the MB release. After the load of MB, the CD-Au blocks the pores of the MBI-MS by the host-guest interaction in the neutral condition. However, when the pH is below 7.0, CD-Au is separated from the surface of MBI-MS owing to the protonation of the aromatic amines. The encapsulated MB is released from the pores of MBI-MS and detected by square wave voltammetry. The controlled release immunosensor shows a relatively wide linear range from 0.001 to 20 ng·mL ' '1 with a low detection limit of 0.25 pg·mL ' '1. The immunosensor also shows good reproducibility and selectivity, which endows it broad application prospect in clinical research.
CITATION STYLE
Ma, H., Wang, Y., Wu, D., Zhang, Y., Gao, J., Ren, X., … Wei, Q. (2016). A Novel Controlled Release Immunosensor based on Benzimidazole Functionalized SiO 2 and Cyclodextrin Functionalized Gold. Scientific Reports, 6. https://doi.org/10.1038/srep19797
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