Here we present an optimized protocol for immunolocalization of meiotic proteins during female meiosis in whole mount tissues. It ensures ovule morphology integrity and homogeneous reagent penetration. The method relies on paraformaldehyde tissue fixation, polyacrylamide embedding, tissue permeabilization, antibody incubation, counterstaining, and confocal microscopy analysis. This protocol has been used in diverse Arabidopsis ecotypes and in the legume Vigna unguiculata.
CITATION STYLE
Gordillo, S. V. G., Escobar-Guzman, R., Rodriguez-Leal, D., Vielle-Calzada, J. P., & Ronceret, A. (2020). Whole-Mount Immunolocalization Procedure for Plant Female Meiocytes. In Methods in Molecular Biology (Vol. 2061, pp. 13–24). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9818-0_2
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