Protein microarray is the next grand challenge after the completion of the human genome project. A key factor critical to the success of protein microarray technology immobilization strategies. Ideally, the immobilization chemistry of a protein microarray should be highly specific for the target protein to be captured. On top of that, the immobilization reaction should also preserve the functional integrity of the immobilized proteins with defined orientation for protein target interaction. Herein, we describe several strategies that allow for highly stable, yet site-specific immobilization of proteins in microarray.
CITATION STYLE
R.Lue, Y.-P., Yeo, S.-Y. D., Tan, L.-P., Chen, G. Y. J., & Yao, S. Q. (2005). Site-Specific Immobilization of Proteins in a Microarray. In The Proteomics Protocols Handbook (pp. 743–751). Humana Press. https://doi.org/10.1385/1-59259-890-0:743
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