In the recent years, genetic engineering of polyamine biosynthetic genes has provided evidence for their involvement in plant stress responses and different aspects of plant development. Such approaches are being complemented with the use of reverse genetics, in which mutants affected on a particular trait, tightly associated with polyamines, are isolated and the causal genes mapped. Reverse genetics enables the identification of novel genes in the polyamine pathway, which may be involved in downstream signaling, transport, homeostasis, or perception. Here, we describe a basic protocol for the generation of ethyl methanesulfonate (EMS) mutagenized populations of Arabidopsis thaliana for its use in reverse genetics applied to polyamines.
CITATION STYLE
Atanasov, K. E., Liu, C., Tiburcio, A. F., & Alcázar, R. (2018). Generation of EMS-mutagenized populations of Arabidopsis thaliana for polyamine genetics. In Methods in Molecular Biology (Vol. 1694, pp. 343–346). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7398-9_29
Mendeley helps you to discover research relevant for your work.