Strategies for high-density cultivation of bio-inoculants in submerged culture with special reference to pseudomonads

Abstract

This chapter provides a brief overview of submerged cultivation methodologies of Pseudomonas-based microbial inoculants, which when delivered as a formulation improves the health of the host plants. The classifications of bio-inoculant delivery systems in various agronomical applications are discussed in the initial section of the chapter. Among various modules in the supply chain of bio-inoculant development, the chapter deals with medium development and cultivation strategies for successful production of active ingredients. The chapter specifically explores the mass propagation strategies of two potential Pseudomonas strains in submerged cultivation with emphasis on fed-batch mode of cultivation.