The Hippo tissue growth control and regeneration pathway is a main regulator of the YAP/TAZ effectors. In this regard, the LATS/NDR serine/threonine protein kinases can function as central components of the Hippo core module. More specifically, LATS/NDR-mediated phosphorylation of YAP/TAZ on different residues can regulate the subcellular localization and/or stability of YAP/TAZ. Therefore, the assessment of LATS/NDR activities can serve as readout for the activity status of the Hippo pathway. Here, we describe our preferred methodology regarding the measurement of the activities of LATS/NDR kinases.
CITATION STYLE
Hergovich, A. (2019). Measuring the Kinase Activities of the LATS/NDR Protein Kinases. In Methods in Molecular Biology (Vol. 1893, pp. 305–317). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8910-2_23
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