Governance of Urban Sustainability Transitions

Abstract

Natural and artificially induced mutants have provided valuable resources for plant genetic studies and crop improvement. In this study, we investigated the genetic and molecular basis of the purple sheath trait in a somaclonal mutant Z418, which was regenerated from a green sheath rice variety C418 through tissue culture. The purple sheath trait in Z418 was heritable and stable based on our 10 years of evaluation. Genetic analysis revealed that the purple sheath trait of the mutant was controlled by a single dominant gene. To map the gene, we scored 89 polymorphic SSRs markers in a F(2) population of 232 plants derived from a cross between Z418 and HX-3, an indica variety with green sheath trait. The gene was initially mapped to the short arm of chromosome 6 between two SSR markers, RPM5 and RM402, with a genetic distance of 1.1 and 10.3 cM, respectively. Thirty-one SSR and indel markers located within the target region were further used to fine-map the gene to a 153-kb interval between two SSR markers (RPM8 and RPM11). The OsC1 gene, which locates within the region and encodes a MYB family transcription factor, was chosen as the candidate gene controlling the purple sheath trait in Z418. Sequencing analysis revealed that OsC1 gene and its transcript in Z418 was 34 bp longer than that in C418. The possible mechanisms for the gene mutation, the developmental and tissue-specific expression of purple anthocyanin pigmentation in Z418, were finally discussed.