D-serine is toxic to plants. D-serine ammonia lyase, which is encoded by the dsdA gene, can attenuate this toxicity with high specificity. In the present study, we explored the function of codon-optimized dsdA with tobacco plastids and rice nuclear transformation system. It was shown that dsdA gene was site-specifically integrated into the tobacco plastid genome and displayed a high level of expression. Genetic analysis of the progenies showed that dsdA gene is maternally inherited and confers sufficient D-serine resistance in tobacco. The effective screening concentrations of D-serine for seed germination, callus regeneration and foliar spray were 10, 30, and 75 mM, respectively. In addition, calluses from homozygous transgenic rice lines also showed significant tolerance to D-serine (up to 75 mM). Our study proves the feasibility of using dsdA gene as a selectable marker in both plastid and nuclear transformation systems.
CITATION STYLE
Li, Y., Wang, R., Hu, Z., Li, H., Lu, S., Zhang, J., … Zhou, F. (2016). Expression of a codon-optimized dsdA gene in tobacco plastids and rice nucleus confers D-serine tolerance. Frontiers in Plant Science, 7(MAY2016). https://doi.org/10.3389/fpls.2016.00640
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