Here we describe a collection of methods that have been adapted to produce highly efficient nuclear and cytoplasmic extracts from adenovirus-infected HeLa cells. We describe how to produce extracts from virus-infected cells and how to analyze RNA splicing in vitro using T7 RNA polymerase-derived splicing substrate RNAs.
CITATION STYLE
Mühlemann, O., & Akusjärvi, G. (2007). Preparation of soluble extracts from adenovirus-infected cells for studies of RNA splicing. Methods in Molecular Medicine, 131, 33–46. https://doi.org/10.1007/978-1-59745-277-9_3
Mendeley helps you to discover research relevant for your work.