Prohibitin as a novel autoantigen in rheumatoid arthritis

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Abstract

The aim of this study was to verify whether prohibitin is a novel autoantigen in rheumatoid arthritis. Material and methods: First, recombinant human prohibitin (rhPHB) protein was cloned, expressed, and purified. Then the anti-prohibitin autoantibodies were detected by western blotting by using rhPHB protein to incubate sera from patients with rheumatoid arthritis (RA). Next, immunoprecipitation was employed to further illustrate whether anti-prohibitin antibodies exist in RA patients. And finally, autoantibodies against the rhPHB protein were investigated using a homemade ELISA kit through the assessment of 258 real clinical samples. Results: It was revealed that anti-prohibitin antibodies existed in the sera of patients with RA. Reactivity of serum IgG against rhPHB was detected in 26 of 86 RA patients (30.3%), 7 of 86 systemic lupus erythematosus (SLE) patients (8.1%), and 1 of 86 apparently healthy donors (HC) (1.2%). Conclusions: Prohibitin was proved to be a novel autoantigen and the corresponding anti-prohibitin autoantibodies were present in the RA patients' blood circulation.

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APA

Shi, L. L., Chen, P., Xun, Y. P., Yang, W. K., Yang, C. H., Chen, G. Y., & Du, H. W. (2015). Prohibitin as a novel autoantigen in rheumatoid arthritis. Central European Journal of Immunology, 40(1), 78–82. https://doi.org/10.5114/ceji.2015.50837

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