Functional analysis by inducible RNA interference in Drosophila melanogaster.

Abstract

Ribonucleic acid (RNA) interference triggered by double-stranded RNA has become a powerful tool for generating loss-of-function phenotypes. It is used to inactivate genes of interest and represents an elegant approach to genome functional analysis by reverse genetics. In Drosophila, RNA interference has been used in both cell culture and animals. We have adopted this approach to reveal the physiological roles of a number of proteins involved in mitochondrial deoxyribonucleic acid metabolism, and present here experimental schemes to induce the stable expression of double-stranded RNA in Schneider cells and in transgenic Drosophila.