Intracytoplasmic sperm injection in cattle

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Abstract

Intracytoplasmic sperm injection (ICSI) involves the microinjection of sperm into a matured oocyte. Although this reproductive technology is successfully used in humans and many animal species, the efficiency of this procedure is low in the bovine species mainly due to failed oocyte activation following sperm microinjection. This review discusses various reasons for the low efficiency of ICSI in cattle, potential solutions, and future directions for research in this area, emphasizing the contributions of testis-specific isoforms of Na/K-ATPase (ATP1A4) and phospholipase C zeta (PLC ζ). Improving the efficiency of bovine ICSI would benefit the cattle breeding industries by effectively utilizing semen from elite sires at their earliest possible age.

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Unnikrishnan, V., Kastelic, J., & Thundathil, J. (2021, January 1). Intracytoplasmic sperm injection in cattle. Genes. MDPI AG. https://doi.org/10.3390/genes12020198

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