Cloning of the transmembrane glycoproteins G and F from a Brazilian isolate of bovine respiratory syncytial virus in a prokaryotic system

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Abstract

The aim of this work was the cloning of those transmembrane glycoproteins G and F from an isolate bovine respiratory syncytial viruses (BRSV) - a Brazilian isolate of BRSV, named BRSV-25-BR in previous studies, in a prokaryotic system to proceed the sequencing of larger genomic fragments. The nucleotide substitutions were confirmed and these clones may also be used in further studies regarding the biological effects of those proteins in vitro and in vivo.

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Silva, L. H. A., Cardoso, K. C., Silva, M. J., Spilki, F. R., & Arns, C. W. (2011). Cloning of the transmembrane glycoproteins G and F from a Brazilian isolate of bovine respiratory syncytial virus in a prokaryotic system. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 63(3), 552–558. https://doi.org/10.1590/S0102-09352011000300003

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