urification of Avian IgY with Trichloroacetic Acid (TCA)

Abstract

By using trichloroacetic acid (TCA) precipitation to separate egg yolk proteins, mainly IgY was demonstrated that this procedure yield twice more IgY that its counterpart polyethylene glycol (PEG). An ELISA demonstrated that it does not affect the functionality of the IgY molecule. The TCA precipitation procedure provides an efficient and rapid way to purify IgY antibody. It can be used for large scale production of high quality IgY.