Isolation of Iindigenous arbuscular mycorrhizal fungi (AMF) to support revegetation on the nickel post-mining land

Abstract

The revegetation on the nickel post-mining area requires symbiotic associations with mycorrhizal fungi to support the plant growth because it faces major obstacles of harsh soil condition. The study was conducted to identify the AMF status on the nickel post-mining land and its association with pioneer plant roots. Soil and root sampling werewas collected on 4 type areas of nickel post-mining land i.e TR (top soil + revegetation), TNR (top soil + no revegetation), NTR (no top soil + revegetation) and NTNR (no top soil + no revegetation) to determine spore density, soil chemical properties and AMF colonization. The results showed that the nickel mining activities interfere in soil fertility and soil microbial population. It was denoted by lack available nutrient and low AMF diversity. Population of AMF only 7 - 83 spore per gram soil. Glomus or Acaulospora is dominating genus in each area. However, AMF occurrence is a vital to help the pioneer plants growth. It was proven by 163 species of pioneer plants that colonize nickel post-mining land associated with the AMF in their root system. Grass species have highest AMF colonization level. Therefore, inoculation of AMF indigenous isolate is needed as an intervention ion post-mining reclamation effort, so the possibility of symbiosis between plant roots and AMF becomes increasing. Eventually the development of natural plants will be accelerated to catalytic the natural succession process.