Plants display a high diversification degree of the SUMO conjugation machinery, which could confer a biological specialization of the different isoforms. For instance, the two essential Arabidopsis SUMO isoforms, SUMO1/2, display the highest conjugation rate when compared to SUMO3 and 5, suggesting that their specific biochemical properties may be linked to their biological specialization. In order to study the biochemical properties of plant SUMO conjugation systems, quantitative biochemical assays must be performed. We will present a detailed protocol for reconstituting an in vitro SUMO conjugation assay covering all steps from protein preparation to assay development.
CITATION STYLE
Castaño-Miquel, L., & Maria Lois, L. (2016). Kinetic analysis of plant SUMO conjugation machinery. In Methods in Molecular Biology (Vol. 1450, pp. 107–123). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3759-2_9
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