Comparison of EB and Fraser enrichment broths for the detection of Listeria spp. and Listeria monocytogenes in raw-milk dairy products and environmental samples

Abstract

This study is a comparison of the isolation frequency of Listeria spp. and Listeria monocytogenes from selected naturally contaminated dairy products, especially soft smear ripened cheeses from raw milk and samples of feces and rinse samples from the udder taken on the farm, by using an enrichment broth (EB) recommended by the International Dairy Federation and the U.S. Food and Drug Administration (IDF and FDA) or Fraser broth as the selective enrichment. Detection and identification were carried out according to the IDF protocols and a polymerase chain reaction technique. Listeria spp. were detected in 39.8% of the 570 samples while 15.3% were positive for L. monocytogenes. For cheese and curd samples. Fraser enrichment broth gave a statistically significant higher recovery for all Listeria spp. (26 to 21%) as well as for L. monocytogenes in particular (9 to 1.4%). For raw milk and samples taken from feces and the udder rinse no significant difference was found between EB and Fraser broth. A combination of both enrichments resulted in an increase of recovery over all matrices by 15%.