Development of extraction system by using thermo-responsive polymer for Bovine Serum Albumin recovery

Abstract

Thermo-responsive polymers, Dehypon®LS54 and Berol®370 were paired randomly with starch derivatives, K4484®dextrin and N-Lite®D maltodextrin for develop thermoseparating ATPS. The developed systems are DehyponLS54/K4484, DehyponLS54/N-Lite.D and Berol370/K4484. All systems were tested with partitioning of BSA as model protein. Among the tested systems, DehyponLS54/K4484 was found as most suitable system for BSA recovery. A small electrochemical potential effect was observed in DehyponLS54/K4484 dextrin system in which the change of pH (pH 8 to 6) had slightly influence the partitioning behavior of BSA. At both pH, BSA was observed had high affinity to starch enriched phase and it caused a low yield value of BSA after thermoseparation step. However, recovery of BSA in water phase at pH 6 was higher 40% compared to pH 8. The addition of Na2SO4 has lowered the cloud point of Dehypon®LS54. © 2009 Asian Network for Scientific Information.