In vivo overproduction of tRNA chimeras yields an RNA insert within a tRNA scaffold. For some applications, it may be necessary to discard the scaffold. Here we present a protocol for selective cleavage of the RNA of interest from the tRNA scaffold, using RNase H and two DNA oligonucleotides. After cleavage, we show that the RNA of interest can be isolated in a one-step purification. This method has, in particular, applications in structural investigations of RNA. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Ponchon, L., Beauvais, G., Nonin-Lecomte, S., & Dardel, F. (2012). Selective RNase H cleavage of target RNAs from a tRNA scaffold. Methods in Molecular Biology, 941, 9–18. https://doi.org/10.1007/978-1-62703-113-4_2
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