Assessment of immunomodulatory effect of natural antioxidants – an in vitro study

Abstract

Aim: To prepare herbal formulation of garlic, turmeric, honey and spirulina and analyse its immunomodulatory effect. Garlic has anti allergic, antitumor effects and chemopreventive effects.Turmeric has analgesic, anti-inflammatory, wound healing and immunomodulatory activities. Spirulina has antioxidant, immunomodulatory, anti-inflammatory, anticancer, anti-viral, and antibacterial effects. Honey has antimicrobial, antioxidant, anticancer, anti-inflammatory. Materials and methods: Garlic, turmeric, honey and spirulina were mixed with 100 ml of distilled water and boiled for 15 minutes, filtered and again concentrated to 5 ml. The immunomodulatory effect was assessed by diphenyl 1-picryl-hydrazylhydrate assay (DPPH), Ferric reducing antioxidant power assay (FRAP) and H2O2 assay. Results: The prepared extract showed significant results at 50 l when assessed using DPPH, FRAP and H2O2 assay (i.e) 72.1%, 94.6% and 67.9% respectively. Conclusion: This study shows the combination of Garlic, turmeric, honey and spirulina formulation have good immunomodulatory effects when compared to the standard. Hence in future it can be used as a safe alternative instead of commercially available medicines in the form of in-situ gel for management of immune mediated disorders.