Measurement of multiple analytes can provide increased sensitivity and specificity for the detection and management of disease. The enzyme-linked immunosorbent assay (ELISA) is currently the "gold standard" for protein quantification; however, individual assays for each analyte must be performed, placing demand on sample volume. On the contrary, multiplex assays using microsphere-based technologies allow for multiple analytes to be simultaneously assayed within a single sample. Here, we present a protocol for the preparation and development of a multiple-analyte assay in human plasma using the BioPlex 200 platform (Bio-Rad), which incorporates xMAP technology (Luminex). © 2011 Springer Science+Business Media, LLC.
CITATION STYLE
Bernhard, O. K., Mathias, R. A., Barnes, T. W., & Simpson, R. J. (2011). A fluorescent microsphere-based method for assay of multiple analytes in plasma. Methods in Molecular Biology, 728, 195–206. https://doi.org/10.1007/978-1-61779-068-3_12
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