The generation of patient-specific induced pluripotent stem (iPS) cells permits the development of next-generation patient-specific systems biology models reflecting personalized genomics profiles to better understand pathophysiology. In this chapter, we describe how to create a patient-specific iPS cell line. There are three major steps: (1) performing a skin biopsy procedure on the patient; (2) extracting human fibroblast cells from the skin biopsy tissue; and (3) reprogramming patient-specific fibroblast cells into the pluripotent stem cell stage.
CITATION STYLE
Li, Y., Nguyen, H. V., & Tsang, S. H. (2016). Skin biopsy and patient-specific stem cell lines. In Methods in Molecular Biology (Vol. 1353, pp. 77–88). Humana Press Inc. https://doi.org/10.1007/7651_2015_225
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