In the last 10 years, imaging assays based on resonance energy transfer (RET) and protein fragment complementation have made it possible to study interactions between components of G protein-coupled receptor (GPCR) signalling complexes in living cells under physiological conditions. Here, we consider the history of such approaches, the current tools available and how they have changed our understanding of GPCR signalling. We also discuss some theoretical and methodological issues important when combining the different types of assay.
CITATION STYLE
Pétrin, D., & Hébert, T. E. (2011). Imaging-Based Approaches to Understanding G Protein-Coupled Receptor Signalling Complexes. In Methods in Molecular Biology (Vol. 756, pp. 37–60). Humana Press Inc. https://doi.org/10.1007/978-1-61779-160-4_2
Mendeley helps you to discover research relevant for your work.