The goldengate genotyping assay: Custom design, processing, and data analysis

Abstract

The Illumina GoldenGate Assay is a technique that is widely used in molecular genetics to analyze up to thousands of single nucleotide polymorphism (SNPs) simultaneously, providing data of very high quality in a fast and efficient manner. This technique allows the user to optimize the number of genetic loci to be interrogated in a way that best suits their research goals. Here are described in detail all the steps to be followed in the process of genotyping a custom panel, from panel design through data analysis. © Springer Science+Business Media, LLC 2013.