In recent yearshuman pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have emerged as a vital cell source for in vitro modeling of genetic cardiovascular disorders, drug screening, and in vivo cardiac regeneration research. Looking forward, the ability to efficiently cryopreserve hPSC-CMs without compromising their normal biochemical and physiologic functions will dramatically facilitate their various biomedical applications. Although working protocols for freezing, storing, and thawing hPSC-CMs have been established, the question remains as to whether they are optimal. In this chapter, we discuss our current understanding of cryopreservation appertaining to hPSC-CMs, and proffer key questions regarding the mechanical, contractile, and regenerative properties of cryopreserved hPSC-CMs.
CITATION STYLE
Preininger, M. K., Singh, M., & Xu, C. (2016). Cryopreservation of human pluripotent stem cell-derived cardiomyocytes: Strategies, challenges, and future directions. In Advances in Experimental Medicine and Biology (Vol. 951, pp. 123–135). Springer New York LLC. https://doi.org/10.1007/978-3-319-45457-3_10
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