Here, we present two approaches to map DNA double-strand breaks (DSBs) and single-strand breaks (SSBs) in the genome of human cells. We named these methods respectively DSB-Seq and SSB-Seq. We tested the DSB and SSB-Seq in HCT1116, human colon cancer cells, and validated the results using the topoisomerase 2 (Top2)-poisoning agent etoposide (ETO). These methods are powerful tools for the direct detection of the physiological and pathological “breakome” of the DNA in human cells.
CITATION STYLE
Baranello, L., Kouzine, F., Wojtowicz, D., Cui, K., Zhao, K., Przytycka, T. M., … Levens, D. (2018). Mapping DNA breaks by next-generation sequencing. In Methods in Molecular Biology (Vol. 1672, pp. 155–166). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7306-4_13
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