Western blot analysis is widely used for detecting protein expression, analysis of protein–protein interactions, and searching for new biomarkers. Also, it is a diagnostic tool used for detection of human diseases and microorganism infections. Some Streptococcus pneumoniae proteins are important virulence factors and a few of them are diagnostic markers. Here, we describe the detection of two pneumococcal proteins, pneumolysin and PpmA, in human urine by using monoclonal and polyclonal antibodies.
CITATION STYLE
Cima-Cabal, M. D., Vazquez, F., de los Toyos, J. R., & del Mar García-Suárez, M. (2019). Protein Expression Analysis by Western Blot and Protein–Protein Interactions. In Methods in Molecular Biology (Vol. 1968, pp. 101–111). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9199-0_9
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