Molecular detection of Brucella spp. from broth culture of clinical samples in Nigeria: Its role in vaccine quality control

Abstract

PCR was employed to detect Brucella spp. from broth cultures of clinical samples using a group specific primer based on IS6501 sequence. The sensitivity and specificity of this assay was confirmed by Southern hybridization analysis using a digoxigenin-labeled DNA probe while reproducibility of the analysis was confirmed by repetition of the test. Also, ERI1 and ERI2 primers were used to differentiate Brucella abortus strain 19 from other strains and the relevance in quality control of Brucella vaccine production highlighted. © 2009 Academic Journals.