Rhizosphere mediated nutrient management in allium hookeri thwaites by using phosphate solubilizing rhizobacteria and tricalcium phosphate amended soil

Abstract

This work describes integrated nutrient management for cultivation of Allium hookeri by using phosphate solubilizing bacteria (PSB) applied in rhizosphere, along with tricalcium phosphate (TCP). Arthrobacter luteolus S4C7, Enterobacter asburiae S5C7, Klebsiella pneumoniae S4C9, S4C10 and S6C1, and K. quasipneumoniae S6C2, were isolated from rhizosphere of Allium hookeri Thwaites, and were found to release substantial amount of soluble phosphate (124.8–266.4 μg/mL) from TCP in vitro conditions. These isolates were experimented for plant growth promoting attributes, including IAA, siderophore, and nitrogen-fixation. Treatment with PSB resulted in enhanced growth of A. hookeri Th., which was even better with TCP amendment with PSB. K. quasipneumoniae S6C2 resulted in 39.1% and 533.3% increase (p ≤ 0.05) of root length and weight respectively. The treatment with these isolates, in TCP amended soil also resulted in 200–250% increase in available P in soil, which was maximum for K. quasipneumoniae (1.866 mg/g).